DTT FAQS, DIOTHIOTHREITOL TOP 20 QUESTION & ANSWERS
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6450 Lusk Blvd. Suite E102
San Diego, CA 92121
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DTT, DITHIOTHREITOL, Cleland’s Reagent FAQS (Frequently Asked Questions)
For the last 15 years, A.G. Scientific has been a leading supplier of DTT, dithiothreitol, historically known as Cleland’s Reagent. We offer a range of catalog sizes, as well as, multi-kilogram quantities for bulk applications. we provide a full service of bottling, sterile formulations, custom packaging, as well as, comprehensive private labeling capabilities.
DTT, is a product for which we often get questions on the following: solution preparation, storage, stability, handling, trouble shooting, how to avoid common mistakes…. Here are the most Frequently Asked Questions (FAQS) we have received from our customers and the appropriate answers. A Material and Safety (MSDS) document link is also available for download.
QUESTION: WHAT IS DTT?
ANSWER: is a protective agent for reducing –S-S TO SH groups. Used as a strong reducing agent for proteins and enzymes. Enhances DNA polymerase activity.
QUESTION: Are there alternative names?
ANSWER: Yes, DTT is also known as Dithiothreitol or originally as Cleland’s reagent after discover, W.W. Cleland, University of Wisconsin.
QUESTION: What is the stability of DTT?
ANSWER: When refrigerated 2-8ºC, it is stable up to 3 years?
QUESTION: How long is it stable if shipped at room temperature?
ANSWER: We can ensure stability ≤ 72 hours without significant degradation or oxidation.
QUESTION: Does material need to be packaged under inert gas?
ANSWER: Yes, argon preferred nitrogen also suitable.
QUESTION: Does DTT show degradation when stressed at 30ºC?
ANSWER: Yes, DTT demonstrates degradation starting after 3 days and increasing rapidly after 5 days.
QUESTION: What are the basic chemical data:
ANSWER: Chemical Formula: C4H10O2S2, CAS Number:3483-12-3; EC Number (EINECS): 222-468-7.
QUESTION: What is the redox potential?
ANSWER:-0.33 V at pH 7.
QUESTION: Is the reducing power limited?
ANSWER: Yes, the reducing power is limited to pH values >7.
Interested in the complete DTT, DITHIOTHREITOL, aka CLELAND'S REAGENT FAQ'S?
INCLUDED IS THE FOLLOWING:
- Consolidated FAQS gathered in a single document.
- Trouble Shooting Guide included.
- MSDS included in FAQs document.
- Stability,Handling, Disposal tips.
CLICK BELOW for complete DTT, DITHIOTHREITOL FAQ'S
Sincerely, Chip Lindgren, Founder & President, A.G. Scientific, Inc.
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A.G. Scientific, Inc., an ISO 9001:2008 Company
6450 Lusk Blvd. Ste. E102,
San Diego, CA 92121, Phone +858 452 9925,
Fax +858 452 9926, www.agscientific.com
Personal email: chip@agscientific.com
Corporate email: info@agscientific.com
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