G-418 FAQS (Frequently Asked Questions)
|
6450 Lusk Blvd. Suite E102
San Diego, CA 92121
|
For the last 15 years, A.G. Scientific has been a leading manufacturer, and supplier of g-418, Geneticin®. Our success with a combination of fermentation & synthesis that has allowed us to build a catalog of over 160 antibiotics and a customer base of researchers, catalog biochemical distributors and cell media manufacturers worldwide. Click here for our Comprehensive Antibiotic E-Guide. We offer the full range of services: custom bottling, sterile formulations, custom packaging, as well as, a full suite of private labeling capabilities.
The popular selection antibiotic, G-418, is a product we often get questions on solution storage & what are the procedures to determine toxic concentrations. Here are the most Frequently Asked Questions (FAQS) we have received from our customers and answers. A Material and Safety (MSDS) document link is also available for download.
- QUESTION: G-418 works on what cell types?
- ANSWER: G-418 inhibits prokaryotic and eukaryotic cell’s protein synthesis. G-418 is toxic to bacteria, yeast, protozoans, higher plant, and mammalian cells.
- QUESTION: What is the working concentration for selection?
- ANSWER: The working concentration for the purpose of selection varies with cell type, media, growth conditions and cell metabolic rate. Recommended concentration for the selection of resistant cells is 10-2000 mg/ml. Commonly used concentrations for selection are 100-2000 mg/ml for mammalian cells, 10-100 mg/ml for plant cells, 500-1000 mg/ml for yeast, and 10-100 mg/ml for Dictyostelium amoebae. Your optimum concentration should be tested experimentally.
- QUESTION: Is G-418 the same as Geneticin®?
- ANSWER: G-418 and Geneticin are the same chemical structure. G-418 is the generic chemical while Geneticin® is a registered trademark brand of LifeTech Corporation.
- QUESTION: What is the potency specification for G418 liquid and powder??
- ANSWER:The potency specification is > 700 µg/mg. Small lot-to-lot variances do occur.
Interested in the complete g418, geneticin FAQ'S?
INCLUDED IS THE FOLLOWING:
- Consolidated FAQS gathered in a single document.
- Trouble Shooting Guide included.
- MSDS included in FAQs document.
- Selection & Maintenance tips.
- Information on performing Dose Response curve.
CLICK BELOW for complete g418, geneticin, FAQ'S
® Geneticin is a registered trademark of Life Tech Corporation
Sincerely, Chip Lindgren, Founder & President, A.G. Scientific, Inc.
************************************************************
A.G. Scientific, Inc., an ISO 9001:2008 Company
6450 Lusk Blvd. Ste. E102,
San Diego, CA 92121, Phone +858 452 9925,
Fax +858 452 9926, www.agscientific.com
Personal email: chip@agscientific.com
Corporate email: info@agscientific.com
************************************************************