Selecting Reagents for Protein Purification, Part 1
|
6450 Lusk Blvd. Suite E102
San Diego, CA 92121
|
Reagents in Protein Purification, Part 1
The key to successful protein purification is choosing the correct process and the correct media and reagents. Although purification can sometimes be a simple one-step precipitation, multiple step processes are more frequently required to attain the desired purity. Your desired purity level will depend on the function to be served by the purified protein. Proteins for therapeutic use and in vivo studies generally require the highest purity level (> 99%), some characterization methods may require purity on the order of 95%-99%, while certain antibody production and sequencing methods may require just < 95% purity.
Additionally, your purification process will depend largely on the properties of your desired protein and targeted contaminants. Reagents and techniques should be chosen according to your objective in the corresponding step of your purification process, whether the aim is to isolate or purify the target protein.
Biodetergents such as Triton-X, n-Octyl-β-D-Glucopyranoside (SEE BELOW), CHAPS are commonly used for solubilizing, extracting, and purifying membrane proteins.
DOWNLOAD the Biological detergent E-booklet to learn:
- Descriptions of each material
- Funtionality & specific enzymatic activity
- Solubility
- Hydrophobicity & pH
- Easy to Skim comprehensive listings.
For all steps in extracting, isolating and purifying proteins, protease inhibitors such as pepstatin, bestatin (structure below), leupeptin, E-64, AEBSF for aspartic proteases or ETDA for metal-dependent proteases can be used in the buffer solution to inhibit protease 
development and prevent damage to the target protein. The list of available reagents is extensive; a proper choice of high-quality reagents will save time and money in the purification and further downstream research processes. Learn about the A.G. Scientific protease inhibitors in this easy to download 8 page E-booklet.
A.G. Scientific is featuring the world’s largest range of proteomic biomarker stabilization & preservation reagents to the marketplace. Immediate elimination of Proteolytic degradation via the ideal combination of protease inhibitors is absolutely essential for preserving & enhancing the long-term stability of these biomarkers. By downloading this E-booklet you will learn:
- AG Scientific's most popular inhibitors.
- Detailed description of fuctionality.
- Detailed description of unique biomarker targets.
- Comparision summary sheet for quick skim & review.
- Complete listing of Custom Protease inhibitor cocktails.
- Pricing comparisons of the 30+ Ready-to-Use formulations.
- Custom formulations available.
Sincerely, Chip Lindgren, Founder & President, A.G. Scientific, Inc.
************************************************************
A.G. Scientific, Inc., an ISO 9001:2008 Company
6450 Lusk Blvd. Ste. E102,
San Diego, CA 92121, Phone +858 452 9925,
Fax +858 452 9926, www.agscientific.com
Personal email: chip@agscientific.com
Corporate email: info@agscientific.com
************************************************************