A.G. Scientific's Biochemical & Bioprocessing Blog

Protein Solubilizers aka Protein Biodetergents from A.G. SCIENTIFIC

6450 Lusk Blvd. Suite E102 San Diego, CA 92121

 

Chemically and Biologically Pure Protein Biodetergents offered by A.G. Scientific, Inc.

Feature	Benefit
Very low contaminating Aldehyde and Peroxide concentration < 0.1 mM	To maximize yields of biologically active proteins
Extremely low conductivity < 5 µmhos	Prevents problems associated with interfering salts and metal ions
10% solution in sterile water for injection	High purity and ease of dispensing of diluted solutions
Pre-scored 5 ml Amber Ampoules	Small package size with amber ampoules to maximize shelf life
Sterile, U.S.P Grade	To maintain biological purity, Improved stability.
Aseptically manufactured and filled, Large Scale GMP  capabilities	For Research use through therapeutic Production capabilities

Non-Ionic Biodetergents 

Non-ionic detergents contain uncharged, hydrophilic head groups that consist of either polyoxyethylene moieties as in TWEEN and TRITON or glycosidic groups as in octyl glucoside and dodecyl maltoside. In general, non-ionic detergents are better suited for breaking lipid-lipid and lipid-protein interactions than protein-protein interactions. Hence, they are considered non-denaturant and are widely used in the isolation of membrane proteins in their biologically active form. 

The use and performance of a detergent is dependent on a number of general properties such as hydrophobicity, CMC, aggregation number, charge etc…(TABLE-1 : Properties of Non-Ionic Detergents).

Several investigators have reported problems associated with aldehyde and peroxide impurities in polyoxyethylene detergents ^5,6,8. There has also been documented evidence of support of microbial growth and toxic metabolite production in non sterilized aqueous solutions of these detergents.

Our product line of Highly purified, Sterile, 10% aqueous solutions of detergents should offer a definite advantage to those working with sensitive membrane-bound proteins.

 

Stability/Storage/Handling: This product is sterile & stable for 2+ years as supplied. Store in a cool dry place. Protect from light.

TABLE -1: Properties of Non-Ionic Detergents

 

Product	Cat.No.	M.W.a	CMCb (mM)	Agg. No.	Avg. Micellar Wt.	HLB No.	Cloud Point
Protein Solubilizer 80 (10% Soln of Tween 80)	P-1498	1310	0.012	58	76,000	15	-
Protein Solubilizer 20 (10% Soln of Tween 20)	P-1502	1228	0.059	-	-	16.7	-
Protein Solubilizer X-100 (10% Soln of Triton X-100)	P-1500	625	0.2-0.9	140	80,000	13	64ºC
Protein Solubilizer X-114 (10% Soln of Triton X-114)	P-1496	427	0.35	-	-	12.4	22ºC
Protein Solubilizer 35 (10% Soln of Brij-35)	P-1504	1198	0.09	40	48,000	16.9	-
Protein Solubilizer 40 (10% Soln of NP-40)	P-1505	603	0.05-0.3	-	-	13.1	80ºC
Protein Solubilizer Set-1 (5 Sample Set: 2X5 ml each)	P-1503	-	-	-	-	-	-

a) Average molecular weights for detergents composed of mixtures of chain lengths; b) Temperature: 20-25^oC

References

Banerjee, P., et al. (1993). Differential solubilization of membrane lipids by detergents. Arch. Biochem. Biophys. 305, 68.

Neugebauer, J. M. (1990). Detergents: An overview. Methods. Enzymol. 182, 239.

Furth, A. H., et al. (1984). Separating detergents from proteins. Methods. Enzymol. 104, 318.

Hjelmeland, L. M., and Chrambach, A. (1984). Solubilization of functional membrane proteins. Methods. Enzymol. 104, 305.

Ashani, Y. and Catravas, G. (1980). Highly reactive impurities in Triton X-100 and Brij 35: partial characterization and removal. Anal. Biochem. 109, 55-62.

Chang, H. W. and Bock E. (1980). Pitfalls in the use of commercial nonionic detergents for the solubilization of integral membrane proteins: sulfhydryl oxidizing contaminants and their elimination. Anal. Biochem. 104, 112-117.

Helenius, A., McCaslin, D. R., Fries, E., and Tanford, C. (1979). Properties of detergents. Methods. Enzymol. 56, 734-749.

Lever, M. (1977). Peroxides in detergents as interfering factors in biochemical analysis. Anal. Biochem. 83, 274.

Slinde, E., and Flatmark, T. (1976). Effect of the hydrophile-lipophile balance of non-ionic detergents on the solubilization of biological membranes and their integral b-type cytochromes. Biochim. Biophys. Acta. 455, 796.

Helenius, A. and Simons, K. (1975). Solubilization of membranes by detergents. Biochim. Biophys. Acta. 415, 29-79 

Triton is a registered trademark of Union Carbide.

Are you interested in our comprehensive 8-PAGE Biodetergent e-BOOKLET? Click here.

Also available is our COMPREHENSIVE 12-PAGE Protease & Phosphatase Inhibitor cocktails e-Booklet. The premier collection of Protein Preservation & Protein Stabilization reagents. Click here.

 

Sincerely, Chip Lindgren, Founder & President, A.G. Scientific, Inc. 

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A.G. Scientific, Inc., an ISO 9001:2008 Company 

6450 Lusk Blvd. Ste. E102,

San Diego, CA 92121,  Phone +858 452 9925,

Fax +858 452 9926, www.agscientific.com

Personal email: chip@agscientific.com

Corporate email: info@agscientific.com

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