Gene Repair Mechanism May Hold Key to New Cancer Treatment

Posted by Tim DeMartin on Apr 5, 2015 6:45:00 PM

Image:  Transcription Network — guarding the genome by sensing DNA damage. Courtesy of:  [Mats Ljungman & David P. Lane Nature Reviews Cancer 4, 727-737 (September 2004) doi:10.1038/nrc1435]

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A.G. Scientific is a leading provider of antibiotics

Posted by Tim DeMartin on Mar 30, 2015 2:12:58 PM

One of our most popular antibiotics, anisomycin, is a protein and DNA synthesis inhibitor that specializes in inhibiting the 80S ribosome network (eukaryotes). Anisomycin’s properties of inhibition become activated when concentrations are enough to effect greater than 95% of the 80S ribosome protein synthesis. Additionally, Anisomycin works by activating the stress response via the MAP kinase signal transduction pathway.

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Anisomycin Shown to Alter Memory Formation

Posted by Tim DeMartin on Mar 25, 2015 8:04:11 PM

 The broadly held idea that memory formation in the brain is the cause of protein synthesis was challenged in light of data released by US researchers. By injecting a protein synthesis inhibitor, Anisomycin, into the brain, researchers then found they could boost certain levels of neurotransmitters. Specifically, by elevating the levels of noradrenalin, dopamine, and serotonin within the amygdala, researchers produced the desired effect of amnesia in rats [1].

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Polyketide Enzymes Shuffle the Molecular Deck

Posted by Tim DeMartin on Mar 22, 2015 10:31:00 PM

Our current knowledge...

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Anisomycin - Protein Synthesis Inhibitor

Posted by Tim DeMartin on Mar 17, 2015 8:32:18 AM

A.G. Scientific, Inc. is pleased to offer Anisomycin (Product # A-1049), isolated and purified from Streptomyces griseolus.

Anisomycin is a bacterial antibiotic which inhibits protein synthesis by binding to 60S ribosomal subunits and blocking peptide bond formation. This prevents protein elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5

Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2/p38MAPK), kinase subtypes and p46/54JNK6-13

Anisomycin synergizes with growth factors and phorbol esters, and superinduces c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14 

Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15

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Are Circadian Clocks the Basis for Inheritance?

Posted by Tim DeMartin on Mar 5, 2015 8:07:17 AM

Life originated on Earth first because of light, as we discussed last week (How Did Protein Synthesis Originate?). Life originated in the presence of Earth’s day and night cycles that most present-day organisms (from bacteria to plants to animals) come equipped with a rich internal clock system that dictates many different behaviors at different times of the day.

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How did protein synthesis originate?

Posted by Tim DeMartin on Feb 26, 2015 9:26:30 AM

The molecular maps that exist today, the ones that continually improve and become more accurate, already seems inextricably complex (See Also:  New Tissue Atlas Shows Protein Distribution Within the Human Body). We have the concepts of DNA transcription down, and the events of translation are well understood. What can be difficult to imagine is how protein synthesis came to be in the first place when so many interlocking pieces are involved and required. Obviously these systems could not have worked without the apparatus already in place, so how did it all come about?

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Advantages of Labeling with A.G. Scientific Reagents

Posted by Tim DeMartin on Feb 22, 2015 10:57:54 PM

A.G. Scientific, Inc. is excited to be able to offer our clients a large selection of fluorescent dyes, reagents and imaging kits useful in fluorescent microscopy, in situ hybridization, flow cytometry, multi-well assays, content screening, reporter assays, and many more.

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3 Particularly Useful Biochemical Tools

Posted by Tim DeMartin on Feb 13, 2015 1:47:58 PM

In addition to being a supplier of many important amino acids, enzymes and proteins, A.G. Scientific also sells many kinds of small molecules that have important and diverse biological functions.

The major biochemicals which People profile most commonly are proteins obtained from beef, the pancreas, plants, and through fermentation.

Let us briefly survey three particularly useful biochemical tools that could serve as the building blocks of your research.

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The Great, Late Vaccine Debate

Posted by Tim DeMartin on Feb 9, 2015 12:34:47 AM

 The transformation of human health in society by vaccines is probably one of the greatest achievements in science. At the moment, the most obvious and visible signs that the facts are not in clear alignment with the truths presented by science, is the great, late vaccine debate. For better or for worse, much of the public argument over whether vaccines are a good or bad thing is really an argument about the value of the technology, and is not necessarily about the enterprise of science. Walking on the moon was a triumph of world-class engineering, which included nearly two centuries of classical physics distilled out by scientists like my Uncle Frank who helped design the original polymer used as the insulating material for the heat shield – who hadn’t the faintest idea of what it would be like to walk on the moon. Penicillin is another technology made possible by sixty years of scientific research in bacteriology, by the work of scientists who studied, in painstaking detail, the names and identities of bacteria, their habits, their diet and metabolism, just so they could have enough knowledge required to figure out that it takes Streptococcus and Staphylococcus in hand with the Penicillium fungus before you can even begin to consider such things as antibiotics.

 Likewise is the case with vaccines. They represent one aspect of science that may be regarded, one long awaiting day, as the most important advancement for human health. This is the most heart-gut-wrenching news of all (in the Western world, anyway) is that a vast percentage of the population is forgoing basic inoculations out of fear it causes autism in young children. Understandably, many parents are weary from the science and a bit skeptical of the idea that a vaccine’s advertised value actually comes with the protection it provides, in this case, for their child.

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